Versiti - Immune-Mediated Red Cell Destruction | Diagnostic Laboratories | Versiti

Immune-Mediated Red Cell Destruction

Versiti’s five Immunohematology Reference Laboratories specialize in evaluation of autoimmune, DAT negative and drug-induced hemolytic anemias.

Immune mediated red cell destruction is caused by the presence of antibodies to the proteins on the surface of the circulating red cell. These antibodies can be caused by an immune response to previously transfused red cells, an autoimmune response against one’s own red cells, or caused by a rare interaction of various drugs and a patient’s red cells. Once an antibody has been formed, a patient may need to receive red cell blood products that are negative for the antigen in question.

Versiti’s Immunohematology Reference Laboratory provides compatible blood to patients with unique transfusion needs.

Our labs have earned a reputation for solving transfusion problems associated with the diagnosis of patients who develop hemolysis. We specialize in evaluation of autoimmune, DAT negative and drug-induced hemolytic anemias. Each year we receive samples from throughout the country for detection of drug dependent red cell antibodies and Super Coombs testing.

Our laboratories are supported in these activities by its stock of rare reagents, skill, special techniques, and high level of training and experience. As an active participant in the American Rare Donor Program, we maintain a large inventory of rare blood at our five locations.


ABO and Rh

Testing for pretransfusion donor and patient compatibility and to assist in the diagnosis


ABO and Rh - Transplant

Testing for donor and patient compatibility in the context of a organ or bone marrow transplant


ABO Antibody Titer

ABO antibody titration tests for the assessment of prognosis in ABO-incompatible hematopoietic stem cell transplantation or for decision-making in ABO-incompatible kidney transplantation.


Antibody Identification Workup

ABO/Rh, Direct Antiglobulin Test and one Ab ID. Based on results determined by IRL, additional tests may include selected cell panel(s), selected cell screens(s), rare cell panel(s), and pertinent antigen typing(s).


Antibody Titration Workup

Detects the presence and measures the amount of antibodies within a person’s blood. Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn (HDN).


Complete Rh Phenotyping of RBCs

Includes: D, C, c, E, e antigens.


DAT Negative Hemolytic Anemia Evaluation

Includes: ABO/Rh, Direct Antiglobulin Tests (including IgA detection), Elution (routine and enhanced), and antibody detection tests (routine and enhanced). Additional tests as necessary based upon results determined by Immunohematology Reference Lab.


Direct Antiglobulin Test (DAT)

Also known as the direct Coombs test, is used primarily to help determine whether the cause of hemolytic anemia.



Detects harmful antibodies related to a rare disorder called paroxysmal cold hemoglobinuria. Not available as an individual test. Requires initial evaluation at additional cost.


Drug-Dependent RBC Antibody Study

Evaluation of immune hemolysis after exposure to certain drugs is important in order to make the correct diagnosis and to prevent possible future adverse complications.Not available as an individual test. Requires initial evaluation at additional cost.



Used for dissociating an antibody that is attached to the surface of a red blood cell. Used in identification of complicated antibodies, the workup of transfusion reactions or hemolytic disease of the newborn, and the workup of warm autoantibodies.


Red Cell Genotyping Panel

Red Cell Genotyping Panel: 72 PCR-hybridization probes are used in 36 polymerase chain reactions to identify the alleles associated with 44 blood group antigens.


Thermal Amplitude Study

Not available as an individual test. Requires initial evaluation at additional cost.


Weak RhD Analysis Workup

This complex test is ordered to resolve a Rh discrepancy (aka D antigen typing discrepancy). The molecular testing will identify Weak D Types 1, 2, and 3. These Weak D Types can be safely deemed Rh positive.

Diagnostic Laboratories
Diagnostic laboratory services including HLA testing, donor testing, immunohematology reference lab testing, hematology testing and more.
Our Experts
Research investigators, postdoctoral fellows and laboratory staff at Versiti Blood Research Institute and Diagnostic Laboratories.
Access requisitions for Versiti Diagnostic Laboratories.