Flow Cytometry Core Laboratory
Instrumentation and Applications
The Flow Cytometry Core provides data analysis with the following software:
- FCS Express
- CFlow Plus
Analysis workstations and training are available for Flow Core users.
Miltenyi Biotec Magnetic Cell Separation Workstation
This workstation consists of a stand and four separation magnets that can be reserved for use. The usually vast initial cell population can be reduced and enriched for the target cell, with little or no added abuse to the cells. This cuts down sorting time and usually increases viability.
The Flow Core provides these services to all of its investigators and outside users, as capacity permits. Instruments are reserved on a first-come, first-served basis with priority given to Versiti Blood Research Institute (VBRI) investigators. Any new users to the Core are required to attend a free training session. All BD FACSAria sorts are operator assisted and therefore must be scheduled at least 24 hours in advance. As a courtesy, we require 24 hours’ notice to cancel a sort, so that time can be made available to other users.
Please discuss your experimental protocol with us. Sample preparation is always critical to success, and some reagents are not recommended due to optical considerations. We need to determine if there are any special cytometer cleaning requirements. The handling of any infectious or otherwise dangerous materials should be discussed prior to booking time on any system.