Molecular and Single Cell Biology
Molecular and Single Cell Biology
This core operates and maintains equipment needed for preparation, quantitation and analysis of DNA and RNA, DNA sequencing, real-time PCR amplification, and single-cell applications. To reserve one of these instruments for use, choose “Schedule Equipment” and “Molecular and Single Cell Biology” in the iLabs platform. To initiate training for an instrument, please choose the “Request Services,” “Molecular and Single Cell Biology” and “Initiate Request” tabs.
Instrumentation and Services
QuantStudio 6 (96-well plates only)
Real-time quantitative PCR system. Can only run 0.2mL semi-skirted plates. This specific instrument is programed to ONLY run the 96-well heating block. Training required.
QuantStudio 6 (96- and 384-well plates)
Real-time quantitative PCR system. Can only run 0.2mL semi-skirted plates. This specific instrument is programed to run 96-well heating block or 384-well heating blocks. Training required.
Agilent TapeStation
Quantitative, automated electrophoresis instrument for use in quality control of DNA and RNA samples. Emphasis on QC for next-generation sequencing libraries. Training required.
Qubit Flex
Qubit Fluorometers detect dyes bound to nucleic acids or other biomolecules. Used for RNA and DNA quantitation and quality in the preparation of sequencing libraries. Training required.
Next-Seq 500
DNA library sequencing instrument. Reagent kits purchased separately by user. Please coordinate with Brad Best (bjbest@versiti.org) for use of instrument, and the Bioinformatics core (bioinformatics@versiti.org) for data export and management. Training required.
10X Genomics Chromium X
The Chromium X controller is a microfluidics-based system for single-cell capture that runs all 10x single-cell assays in standard, low- and high-throughput modes. Compatible with CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing). Reagent kits and processing plates are purchased. Training required.
BD Rhapsody
The BD Rhapsody Single-Cell Analysis System allows capture of 100-100,000 cells on a microwell-based cartridge for multiomics experiments. Compatible with barcoded antibodies for quantitative detection of surface and intracellular antigens by CITE-Seq. Training required.
Takara ICELL8
The ICELL8 platform enables automated dispensing and isolation of single cells in nanowell cartridges for downstream analysis. The instrument allows visual inspection of isolated single cells to distinguish live from dead cells and identify multinucleated cells. Large-bore nozzle suited for isolation of fragile, large cell types. Compatible with SMART-Seq, ATAC-Seq and TCR profiling. Training required.
Diagenode Bioruptor
Tissue disruption and chromatin shearing in sealed tubes of 0.5-50 mL capacity. Built-in cooling system.
Miltenyi Octo with Heaters
Highly versatile tissue disruption with single-use gentleMACS tubes. Enables generation of homogenates or single-cell suspensions in a closed, sterile system. Eight samples can be individually processed at a user-defined temperature.
Omni Bead Raptor
Tissue disruption bead mill for grinding, lysing and homogenizing biological samples prior to molecular extraction. 0.5-50 mL capacity.