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Histology Core Lab

The Histology Core provides services for tissue sectioning, preparation and staining.

Overview

The Histology Core provides services for tissue sectioning, tissue preparation and tissue staining. Core staff provide services in tissue processing, tissue embedding, sectioning and basic staining procedures. A cryostat is available for frozen section preparation.

Any usage should be scheduled at least 48 hours prior to the time needed.

 

Contact Information

Barb Fleming, BA
Core Laboratory Officer
bafleming@versiti.org
414-456-8179

Accreditations and Licenses

Instrumentation and Applications

Automated Tissue Processor

  • This processor is used for paraffin sections. Tissues are preferred in 10% neutral buffered formalin.

Sakura Automated Stainer

  • The stainer can process 40 slides per run, hematoxylin and eosin (H&E). Dewaxing and hydrating services are available. These are useful when the user’s lab wants to perform its own specialized staining procedures.

Cryostat

  • The cryostat is used for frozen sections. OCT mounting media is available. Frozen sections using Leica’s CryoJane Tape-Transfer System are also available. The CryoJane process is designed specifically to avoid melting and air-drying the associated artifacts.

Manual Staining

  • Stains available include trichrome, iron, periodic acid-Schiff (PAS) and more.

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