HLA Loss of Heterozygosity

HLA LOH assay for patients with post-transplant relapse.

HLA Loss of Heterozygosity (LOH)


The HLA Loss of Heterozygosity Evaluation is intended for detecting and characterizing the presence or absence of genomic loss of heterozygosity in the HLA region for patients with post-transplant relapse. The HLA LOH assay starts with flow cytometric enrichment of relapsing cells, followed by genomic analysis using two different methods to ensure accurate detection of genomic loss.

HLA LOH Testing Approach

  • Flow cytometric analysis and sorting for the enrichment of relapsing cells provides high sensitivity.
  • Two methods for genomic analysis provide spatial information across the MHC region and high-resolution allelic detail across all classical HLA loci for a complete genomic picture applicable in all transplant patient donor pairs. 
  • Versiti provides interpretation on HLA loss in the context of the patient and donor HLA antigens. Loss of the non-shared alleles may indicate a loss of the graft leukemia effect from the transplant.
 
 

Enabling more effective treatment decisions

Versiti’s unique assay starts with flow cytometric enrichment of relapsing cells followed by genomic assay analysis using two different methods to ensure accurate detection of genomic loss.

 
Chimerism Testing for Engraftment
Among Versiti Diagnostic Labs’ transplant testing menu is chimerism testing for post-transplant and cellular therapy patients.
 
Diagnostic Laboratories
Diagnostic laboratory services including HLA testing, donor testing, immunohematology reference lab testing, hematology testing and more.
 
Human Leukocyte Antigen (HLA) Typing
Versiti Diagnostic Labs’ Histocompatibility Lab provides human leukocyte antigen (HLA) typing for solid organ and stem cell transplant patients.
 
Molecular Oncology Testing
Molecular oncology lab tests to help diagnose blood cancers like acute myeloid leukemia, chronic myelogenous leukemia and more.