2722

HLA Loss of Heterozygosity Evaluation

Order Code: 2722 Get Requisition Form
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Test Information
This test is intended for patients with confirmed disease relapse post-hematopoietic cell transplant to evaluate the presence or absence of genomic loss of heterozygosity in the HLA region in malignant cell populations.
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Disease State
Stem Cell Transplantation
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Sample Notes
Na Heparin Whole Blood or Bone Marrow (Green top)
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Requested Volume
2-3ml Na Heparin Bone Marrow or 5-10ml Na Heparin Whole Blood (Green top)
Minimum / Pediatric Volume Icon
Minimum / Pediatric Volume
1ml Na Heparin Bone Marrow or 5ml Na Heparin Whole Blood (Green top)
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Additional Sample Information
Test requires fresh blood or bone marrow with malignant cell counts ideally at 5% or greater. Collect in Na Heparin, store and ship ambient same day. Must be received at Hematologics within 72 hours after collection. Indicate on specimen tube and requisition whether sample is whole blood or bone marrow.
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Shipping Information
Room Temperature
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CPT Codes
  • 88184 (first marker)
  • 88185 (each add’l marker)
  • 88189
  • 81268
  • 81265
  • 81378
  • 81382x5
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Method
Malignant cells are enriched by flow-based cell sorting targeting specific cell surface markers. Genomic DNA from the sorted cells is extracted and analyzed for loss of HLA heterozygosity (LOH) using STR markers and next generation sequencing.
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Turnaround Time
8-12 days
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DEX Z-Code™
No
 
Our Experts
Research investigators, postdoctoral fellows and laboratory staff at Versiti Blood Research Institute and Diagnostic Laboratories.
 
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Versiti Diagnostic Labs’ Histocompatibility Lab provides human leukocyte antigen (HLA) typing for solid organ and stem cell transplant patients.
 
Requisitions
Access requisitions for Versiti Diagnostic Laboratories.