2722
HLA Loss of Heterozygosity Evaluation
Order Code: 2722
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Test Information
This test is intended for patients with confirmed disease relapse post-hematopoietic cell transplant to evaluate the presence or absence of genomic loss of heterozygosity in the HLA region in malignant cell populations.
Disease State
Stem Cell Transplantation
Sample Notes
Na Heparin Whole Blood or Bone Marrow (Green top)
Requested Volume
2-3ml Na Heparin Bone Marrow or 5-10ml Na Heparin Whole Blood (Green top)
Minimum / Pediatric Volume
1ml Na Heparin Bone Marrow or 5ml Na Heparin Whole Blood (Green top)
Additional Sample Information
Test requires fresh blood or bone marrow with malignant cell counts ideally at 5% or greater. Collect in Na Heparin, store and ship ambient same day. Must be received at Hematologics within 72 hours after collection. Indicate on specimen tube and requisition whether sample is whole blood or bone marrow.
Shipping Information
Room Temperature
CPT Codes
- 88184 (first marker)
- 88185 (each addl marker)
- 88189
- 81268
- 81265
- 81378
- 81382x5
Method
Malignant cells are enriched by flow-based cell sorting targeting specific cell surface markers. Genomic DNA from the sorted cells is extracted and analyzed for loss of HLA heterozygosity (LOH) using STR markers and next generation sequencing.
Turnaround Time
8-12 days
New York State Approval
No
DEX Z-Code™
Yes, visit app.dexzcodes.com