Brian Curtis, PhD, D(ABMLI), MT(ASCP) SBB
Brian Curtis, PhD, D(ABMLI), MT(ASCP) SBB
Senior Director, Diagnostic Hematology, Senior Investigator
Director, Platelet & Neutrophil Immunology Lab
Versiti Blood Center of Wisconsin
Versiti Blood Research Institute
Clinical and Translational Science Institute
Medical College of Wisconsin
Education and Training
University of Wisconsin-Milwaukee, PhD, 2010
Medical Technology (MT), American Society of Clinical Pathologists (1986)
Specialist in Blood Banking (SBB) American Society of Clinical Pathologists (1991)
Diplomat, American Board of Medical Laboratory Immunology D(ABMLI) (2012)
The focus of my lab’s research is on detection and characterization of platelet and neutrophil-reactive antibodies and antigens and studies of the glycoproteins that carry these antigens. The primary goal is to apply the results of this work to develop new and improved clinical tests for use by the Diagnostic Laboratories at Blood Center of Wisconsin.
Drug-Dependent Platelet Antibodies
Some people develop antibodies against their platelets, red blood cells, or white blood cells following receipt of medications. Our Platelet & Neutrophil Immunology Reference Lab currently tests for drug-dependent platelet and white blood cell antibodies, and to date, we have identified drug-dependent antibodies in patients’ blood samples for over 60 drugs, including vancomycin, sulfa-drugs, beta-lactam antibiotics, and even over-the-counter medications like ibuprofen and Tylenol (view complete list). We currently have laboratory and clinical studies in progress, together with researchers at BRI, to better understand the formation of drug-dependent antibodies and their clinical significance.
Studies of CD36 on Platelets
We showed that ~3% of African-Americans lack the protein CD36/glycoprotein IV on their platelets. CD36 is a receptor for malaria-infected red blood cells, and absence of the protein from platelets and monocytes may play a role in malaria infection. Studies are ongoing to identify the rate of alloimmunization to CD36 in patients with CD36 deficiency and the role of these antibodies in immune platelet disorders.
Low Frequency Human Platelet Alloantigens (HPA)
The Platelet & Neutrophil Immunology Lab performs testing on over 400 samples each year to aid physicians in the clinical diagnosis of fetal & neonatal alloimmune thrombocytopenia (FNAIT). FNAIT is a disorder in pregnancy in which a mother makes antibodies against her baby’s platelets resulting in destruction of the baby’s platelets leading to severe bleeding into the baby’s brain (ICH) and even death. It is thus important that laboratory testing for FNAIT be thorough, including the ability to detect maternal antibodies reactive with low frequency or new HPA (view list Platelet Antigen Database). We are working with cutting-edge methods to detect and characterize newly discovered HPA.
Leukocyte Antibodies in TRALI
Transfusion-Related Acute Lung Injury (TRALI) is a severe reaction that some patients experience following transfusion of blood or plasma. TRALI is currently the number one cause of transfusion-related death reported to the Food and Drug Administration (FDA). In a significant number of TRALI cases, the plasma from donors of the implicated blood products contains leukocyte antibodies (HLA and neutrophil) that cause activation of the patient’s neutrophils, leading to TRALI. One of the antigens targeted by such antibodies is designated human neutrophil antigen (HNA)-3a, which has been implicated in numerous severe cases of TRALI, many of them fatal. We identified the protein carrier (CTL2) of HNA-3a and HNA-3b, alleles of gene SLC44A2. We are also investigating the use of tests that could be used to screen blood donors for antibodies against HNA-3 as a TRALI risk-reduction measure.
Versiti Moonshot Fund, "Designer Blood Cells Using CRISPR-Cas 9 Gene Editing," Role: PI (2019
Supervisor, Platelet & Neutrophil Immunology Lab
Technical Specialist, Platelet & Neutrophil Immunology Lab
Operations Director, Diagnostic Hematology
- Curtis BR, Edwards JT, Hessner MJ, Klein JP, Aster RH. Blood group A and B antigens are strongly expressed on platelets of some individuals. Blood 2000; 96:1574-1581.
- Aitman TJ, Cooper LD, Norsworthy JP, Wahid FN, Gray JK, Curtis BR, McKeigue PM, Kwiatkowski D, Greenwood BM, Snow RW, Hill AV, Scott J. Malaria susceptibility and CD36 mutation. Nature 2000; 405: 1015-1016.
- Curtis BR, Bussel JB, Manco-Johnson M, Aster RH, McFarland JG. Fetal and neonatal alloimmune thrombocytopenia in pregnancies involving in vitro fertilization: a report of four cases. Am J Obstet and Gynecol 2005; 192:543-547.
- Silliman CC, Curtis BR, Kopko P, Khan SY, Kelher M, Shuler R, Sannoh B, Ambruso DR. Donor antibodies to HNA-3a implicated in TRALI reactions prime neutrophils and cause PMN-mediated damage to human pulmonary microvascular endothelial cells in a two-event in vitro model. Blood 2007; 109:1752-1755.
- Von Drygalski A, Curtis BR, Bougie DW, McFarland JG, Ahl S, Limbu I, Baker KR, Aster RH. Vancomycin-induced immune thrombocytopenia. N Engl J Med 2007; 356:904-910.
- Podrez EA, Byzova TV, Febbraio M, Salomon RG, Ma Y, Valiyaveettil M, Poliakov E, Sun M, Finton PJ, Curtis BR, Chen J, Zhang R, Silverstein RL, Hazen SL. Platelet CD36 links hyperlipidemia, oxidant stress and a prothrombotic phenotype. Nature Medicine 2007; 13:1086-1095.
- Curtis BR, Fick A, Lochowicz, McFarland JG, A, Ball RH, Peterson, JA, Aster RH. Neonatal alloimmune thrombocytopenia (NATP) associated with maternal fetal incompatibility for blood group B. Transfusion 2008; 48:358-364.
- Curtis BR, Cox NJ, Sullivan MJ, Konkashbaev A, Bowens K, Hansen K, Aster RH. The neutrophil alloantigen HNA-3a (5b) is located on choline transporter- like protein 2 (CTL2) and appears to be encoded by an R>Q154 amino acid substitution. Blood 2010; 115:2073-2076.
- Reese JA, Li X, Hauben M, Aster RH, Bougie DW, Curtis BR, George JN, Vesely SK. Identifying drugs that cause acute thrombocytopenia: an analysis using 3 distinct methods. Blood 2010; 116(12):2127-2133.
- Curtis BR, Sullivan MJ, Holyst T, Szabo A, Bougie, DW, Aster RH. A 36-mer peptide from CTL2 containing arginine 154 is recognized by a subset of HNA-3a alloantibodies implicated in TRALI. Transfusion 2011; 51:2168-2174.
- Achterbergh R, Vermeer E, Curtis BR, Porcelijn L, Aster RH, Deenik W, Daemen-Gubbels K. Thrombocytopenia in a nutshell. Lancet 2012; 379:776.
- Bowens K, Sullivan M, Curtis BR. Determination of neutrophil alloantigen HNA-3a and HNA-3b genotype frequencies in six racial groups by high-throughput 5’ exonuclease assay. Transfusion 2012 Nov; 52(11):2368-2374.
- Thomas GM, Carbo C, Curtis BR, Martinod K, Mazo IB, Schatzberg D, Cifuni SM, Fuchs TA, von Andrian UH, Hartwig JH, Aster RH, Wagner DD. Extracellular DNA traps are associated with pathogenesis of TRALI in humans and mice. Blood 2012; 119:6335-6343.
- Curtis BR, McFarland JG. Human platelet antigens - 2013. Vox Sang 2014; 106:93-102.
- Sullivan MJ, Peterson J, McFarland JG, Bougie D, Aster RH, Curtis BR. A new low frequency alloantigen (Khab) located on platelet glycoprotein IIIa as a cause of maternal sensitization leading to neonatal alloimmune thrombocytopenia. Transfusion 2015; 55:1584-1585.
- Arnold DM, Curtis BR, Bakchoul T. Recommendations for standardization of laboratory testing for drug-induced thrombocytopenia. J Thromb Haemost 2015; Apr;13(4):676-678.
- Curtis BR. Recent progress in understanding the pathogenesis of fetal and neonatal alloimmune thrombocytopenia. Br J Haematol 2015; 171, 671–682.
- Curtis BR, Roman A, Sullivan M, Raven C, Larison J, Weitekamp L. Two Cases of Maternal Alloimmunization Against Human Neutrophil Alloantigen-4b, One Causing Severe Alloimmune Neonatal Neutropenia. Transfusion 2016;56:101–106.
- Padmanabhan A, Jones CG, Curtis BR, Bougie DW, Sullivan MJ, Peswani N, McFarland JG, Eastwood D, Wang D, Aster RH. A novel PF4-dependent platelet activation assay identifies patients likely to have heparin-induced thrombocytopenia/thrombosis (HIT). Chest 2016; 150(3):506-15.
- Zhang N, Zhi H, Curtis BR, Rao S, Jobaliya C, Poncz M, French DL, Newman P. Designer Platelets: CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes. Blood 2016; 127(6):675-680.
- Metzner K, Bauer J, Ponzi H, Ujcich A, Curtis BR. Detection and identification of platelet antibodies using a sensitive multiplex assay system - Platelet Antibody Bead Array (PABA). Transfusion Jul 2017; 57(7):1724-1733.
- Sullivan MJ, Kuhlmann R, Peterson J, Curtis BR. A new low frequency alloantigen (Domb) located on platelet glycoprotein IIIa as a cause of maternal sensitization leading to neonatal alloimmune thrombocytopenia. Transfusion Jul 2017; 57(7):1847-1848.
- Curtis BR. Non-chemotherapy drug-induced neutropenia: key points to manage the challenges. Hematology Am Soc Hematol Educ Program. Dec 2017; 2017(1):187-193. doi: 10.1182/asheducation-2017.1.187.
- Curtis BR, Hsu Y-MS, Podoltsev N, Lacy J, Curtis S, DeSimone R, Samuel MS, Bougie DW, Aster RH. Patients treated with oxaliplatin are at risk for thrombocytopenia caused by multiple drug-dependent antibodies. Blood Mar 2018;131(13):1486-1489. doi: 10.1182/blood-2017-10-812461
- Thinn AMM, Wang Z, Zhou D, Zhao Y, Curtis BR, Zhu J. A The autonomous conformational regulation of β3 integrin and the conformation-dependent property of HPA-1a alloantibodies. PNAS Sept 2018; 115(39):E9105-E9114. doi: 10.1073/pnas.1806205115. PMID:30209215
- Zhi H, Ahlen MT, Thinn AMM, Lertkiatmongkol P, Weiler H, Curtis B, Skogen B, Zhu J, Newman PJ. High-resolution mapping of the polyclonal immune response to the human platelet alloantigen HPA-1a (PlA1). Blood Advances Nov 2018; 2(21):3001-3011. doi: 10.1182/bloodadvances.2018023341.
- Petermann R, Bakchoul T, Curtis B, Mullier F, Miyata S, Arnold DM. Investigations for Fetal and Neonatal Alloimmune Thrombocytopenia (FNAIT): Recommendations from the Platelet Immunology Scientific Subcommittee. J Thromb Haemost Dec 2018; (12):2526-2529. doi: 10.1111/jth.14294.
- Zhang N, Santoso S, Aster RH, Curtis BR, and Newman PJ. Bioengineered iPSC-derived megakaryocytes for the detection of platelet-specific patient alloantibodies. Blood 2019; 134(22):e1-e8. doi: 10.1182/blood.2019002225.
- Hawkins J, Aster R, Curtis BR. Post-transfusion purpura: current perspectives. J Blood Med. Dec 2019; 10:405-415. doi: 10.2147/JBM.S189176
- Kjær M, Geisen C, Akkök C, Wikman A, Sachs U, Bussel JB, Nielsen K, Walles K, Curtis BR, Vidarsson G, Skogen B. Strategies to develop a prophylaxis for the prevention of HPA-1a immunization and fetal and neonatal alloimmune thrombocytopenia. Transfus Apher Sci 2020; 59(1):102712. doi: 10.1016/j.transci.2019.102712. Epub 2019 Dec 31.
- Narayanan PK, Curtis BR, Shen L, Schneider E, Tami JA, Paz S, Burel SA, Tai L-J, Machemer T, Kwoh TJ, Xia S, Shattil SJ, Witztum JL, Engelhardt JA, Henry SP, Monia BP, Hughes ST. Underlying immune disorder may predispose some transthyretin amyloidosis subjects to inotersen-mediated thrombocytopenia. Nucleic Acid Ther 2020; Apr;30(2):94-103. doi: 10.1089/nat.2019.0829.
- Kjær M, Geisen C, Akkök ÇA, Wikman A, Sachs U, Bussel JB, Nielsen K, Walles K, Curtis BR, Vidarsson G, Järås K, Skogen B. Strategies to develop a prophylaxis for the prevention of HPA-1a immunization and fetal and neonatal alloimmune thrombocytopenia. Transfus Apher Sci 2020 Feb;59(1):102712. doi: 10.1016/j.transci.2019.102712. Epub 2019 Dec 31.
- Puram RV#, Erdil RM#, Weber BN, Knelson EH, Van Beuningen AM, Wallwork R, Gilyard SN, Curtis BR, Ranganathan R, Leaf RK, Malhotra R. Thrombocytopenia and thromboses in myocardial infarction associated with eptifibatide-dependent activating antiplatelet antibodies. Thromb Haemost 2020 Jul;120(7):1137-1141. doi: 10.1055/s-0040-1712458. Epub 2020 May 29. # Contributed Equally
- Bannow BS, Warad DM, Jones CG, Pechauer SM, Curtis BR, Bougie DW, Sharma R, Grill DE, Redman MW, Khalighi PR, Leger RR, Pruthi RK, Chen D, Sabath DE, Aster RH, Garcia DA, Padmanabhan A. A prospective, blinded study of a PF4-dependent assay for HIT diagnosis. Blood 2021 Feb 25;137(8):1082-1089. doi: 10.1182/blood.2020008195.
- Gemlyn G, Friedman KD, Curtis BR, Lind SE. Successful treatment of thrombotic thrombocytopenia with cerebral sinus venous thrombosis following Ad26.COV2.S vaccination. Am J Hematol 2021 Aug 1;96(8):E301-E303. doi: 10.1002/ajh.26237. Epub 2021 Jun 1.
- Pandey P, Zhang N, Curtis BR, Newman PJ, Denomme GA. Generation of ‘Designer Erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene-edited cultured human induced pluripotent stem cells. J Cell Mol Med 2021 Sept 21. Doi:10.1111/jcmm.16872. Online ahead of print.
- Chaffin J (Host), Curtis BR (Guest). (2017, Feb 13) NAIT (No. 027) [Audio podcast episode] in Blood Bank Guy
- Curtis BR (Speaker). (2017, May 10) Platelet Immunology (Master Program No. 17EL-133) [Professional Development eCast] in AABB Immunohematology Boot Camp
- Curtis BR (Speaker). (2019, March 6) A Newborn Infant has a Low Platelet Count - Important diagnostic features of Neonatal Alloimmune Thrombocytopenia (NAIT) [BloodFLOW (Further Learning On Web) Webinar] in beLearning by Versiti
- Curtis BR, Bakchoul T (Hosts). (2019, February 25) Module 4: Drug-Induced Thrombocytopenia (No. 234427) [ISTH Academy] in Acquired Thrombocytopenia Course
- Sharma R, Curtis BR (Speakers). (2020, July 23) Recent Advances in Diagnosis and Treatment of HIT. [BloodFLOW] in beLearning by Versiti
- 1996 U.S. Serial No. 5,585,243. Inventors - RH Aster and BR Curtis. METHOD OF DETECTING CYTOPENIA THAT IS MEDIATED BY DRUG-DEPENDENT ANTIBODY BINDING TO BLOOD CELLS
- 2015 U.S. Serial No. 9,017,958 B2. Inventor – Brian R. Curtis. METHOD OF SIMULTANEOUS DETECTION OF HEPARIN-INDUCE IMMUNOGLOBULINS TYPES G, A, AND M.